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Romanian Journal of Biology - Plant Biology
ROMANIAN ACADEMY
Institute of Biology -Bucharest
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IMPROVING SOIL FERTILITY THROUGH THE USE OF NOVEL OXIDIC MATERIALS FUNCTIONALIZED WITH HALOPHILIC BACTERIAL ENZYMES
ALINA BADEA (CIOACĂ), SIMONA NEAGU, MĂDĂLIN ENACHE, ROBERT RUGINESCU, MIHAI ANASTASESCU, CRINA ANASTASESCU, SILVIU PREDA, MARIA ZAHARESCU, VERONICA LAZĂR
Abstract:
This study investigates the role of halophilic bacterial enzymes, specifically proteolytic enzymes synthesized by a strain from Movila Miresei Salt Lake, Romania, in enhancing anthropized soil fertility through hybrid structures. Utilizing a moderately halophilic (MH) medium with 10% NaCl and 1% casein, the enzymes were biosynthesized, purified via acetone precipitation and gel filtration, and immobilized on titanium dioxide (TiO2) and silica (SiO2) nanomaterials through direct adsorption. Titanium dioxide demonstrated superior immobilization effectiveness. These enzyme-nanomaterial hybrids were mixed into anthropized soil at a 1:1 ratio. The improvement in soil biodegradation was assessed after 72 hours by measuring activities of alkaline phosphatase, urease, and dehydrogenase in soil samples containing immobilized enzymes and in those with only nanomaterials.
Citation:
ALINA BADEA (CIOACĂ), SIMONA NEAGU, MĂDĂLIN ENACHE, ROBERT RUGINESCU, MIHAI ANASTASESCU, CRINA ANASTASESCU, SILVIU PREDA, MARIA ZAHARESCU, VERONICA LAZĂR, 2024. IMPROVING SOIL FERTILITY THROUGH THE USE OF NOVEL OXIDIC MATERIALS FUNCTIONALIZED WITH HALOPHILIC BACTERIAL ENZYMES, Romanian Journal of Biology - Plant Biology, 69(1-2): 719.
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